Wednesday, May 11, 2011

Flame photometry

Flame photometry is a method used by researchers to detect metal salts. This is an atomic emission method detection. Here metal solutions are initially aspirated into the flame. The hot flame evaporates the solvent and atomises the metal. This atomization excites valence electrons to upper state. When these valence electrons return to ground state, it is associated with a light emission. For monitoring emitted wavelength, optical filters are used. In the design of flame photometer, essential components are burner, nebuliser, monochromator, detector and a recorder. This method is widely used in clinical, biological and environmental analysis and does not produce any hazardous waste. Major application is in quantitative determination of metals in alkali and alkaline earth solutions. Flame temperature here is to be optimum since intensity of emission is very sensitive to changes in flame temperature.

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